Corning® polystyrene microplates are available in a variety of modified surfaces. These surfaces can support binding or covalent immobilization of cells, proteins, nucleic acids, and other biomolecules. Surface options include:
| Microplate Surface Treatment |
Description |
| Not treated (or medium binding) polystyrene surface |
Hydrophobic in nature and binds biomolecules through passive interactions. It is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface. |
| High binding surface |
Capable of binding medium (>10 kD) and large biomolecules that possess ionic groups and/or hydrophobic regions. |
| Nonbinding surface (NBS) |
A Corning® proprietary treatment technology used on polystyrene microplates to create a nonionic hydrophilic surface (polyethylene oxide-like) that minimizes molecular interactions. Ideal for reducing protein and nucleic acid binding at low concentrations, and increasing assay signal to noise. |
| Corning® CellBIND® Surface |
A Corning proprietary treatment which provides improved consistency and even cell attachment. |
| Tissue culture treated (TC-Treated) |
Used for the attachment and growth of anchorage-dependent cells. |
| Ultra-Low attachment surface |
Has a covalently bonded hydrogel designed to minimize cell attachment, protein absorption, enzyme activation and cellular activation. This surface is
noncytotoxic, biologically inert and nondegradable. |
| Poly-D-lysine coated surface |
Can improve attachment of difficult-to-attach cells. |
| Sulfhydryl (Sulfhydryl-BIND™) binding surface |
Has covalently-linked maleimide groups that covalently couple to sulfhydryl groups via SH moieties. Ideal for assays requiring site-directed orientation of a biomolecule, especially antibodies. |
| Carbohydrate (Carbo-BIND™) binding surface |
Hydrazide groups covalently coupled to carbohydrate groups. Ideal for assays requiring site-directed orientation of a biomolecule (oxidized antibodies, carbohydrates, and glycosylated proteins) while maintaining enzymatic or immunological activity. |
| Photo-reactive (Universal-BIND™ surface |
Covalently immobilizes biomolecules via abstractable hydrogens using UV illumination, resulting in a carbon-carbon bond. Although linkage is nonspecific and does not allow for site-directed orientation of a biomolecule, this surface may be useful for immobilization of double stranded DNA, antigens of unknown structure, and mixtures of biomolecules (e.g., cell lysates). |
| Amine surface |
Has positively charged amine groups (2 x 1013 reactive sites/cm2) that can be used for covalent immobilization via bifunctional crosslinkers. |
